siRNA cassette is a PCR product which consists of a promoter and terminator sequence flanking a DNA insert encoding a hairpin siRNA. PCR can be used to rapidly produce different combinations of siRNA template and RNA polymerase promoter. The PCR products are directly transfected into mammalian cells resulting in functional expression of siRNA. This approach is the most cost-effective way for identifying the most potent and specific siRNA target sequences. This service includes constructing the siRNA expression cassette from your siRNA sequences and PCR amplification. At the completion of the project, you will receive 20 ug of siRNA expression cassette and an experimental report.
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