In vitro site-directed mutagenesis is an important methodology used to study protein structure-function relationships, identify intramolecular regions or amino acids that may mediate these functions, analyze gene expression and modify vectors. Many published mutagenesis methodologies are labor intensive and technically difficult to perform. With many years of experience in mutational manipulation, Retrogen has developed its own methodology that allows us to finish any mutagenesis project at very fast turnaround time and at a very affordable price.
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The Benefits of Getting Mutagenesis Projects Done with Retrogen
Get Consultation from Our Experienced Scientists
Get Your Mutagenesis Projects Done Fast at a Very Competitive Price
Sequence the Mutated Region to Ensure a 100% Accuracy
5 µg of Purified Plasmid Delivered is Ready to Use
A Complete Report with Chromatogram Printout
Starting Material:
Please send 2-5 µg of plasmid DNA
Email copies of vector map and sequence of the construct with indication of the bases that need to be changed to gene@retrogen.com
Method:
We use PCR site-directed method in conjunction with our proprietary protocols
We sequence the mutated region by automated fluorescent sequencing
Final Form :
5 µg of lyophilized plasmid DNA with the correct changes
Documentation:
A complete report of strategies and data
A printout of assembly report and chromatograms from automated sequencers
Price
Each mutant is $300 - $500 which includes both strands sequencing verification. Please call us for a discounted price if you have more than one mutant to be made.
Turnaround time
Each mutant will take 2-3 weeks, including sequence verification.
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Ordering
Make sure that the correct changes are indicated on the sequence of the construct Email modified sequence and original sequence to gene@retrogen.com Enclose 20 µg plasmid DNA and vector map Send the package priority overnight to the following address:
Retrogen, Inc
Gene Construction Department
6645 Nancy Ridge Drive
San Diego, CA 92121
858-455-8411 (Tel)
858-455-7987 (Fax) info@retrogen.com (E-mail)
Requirements for Mutagenesis Projects
Complete DNA sequence of the entire plasmid, both the gene and the vector
Indication of codon changes needed (specific individual base changes)
Other changes (deletions, insertions, restriction site changes) clearly indicated
2-5 µg of starting template
Note: If the complete sequence of the plasmid is not known, Retrogen will evaluate the project based on the known sequence and determine whether it should be attempted. If the project cannot be completed, the customer will be billed for the full project price to compensate Retrogen for any time, effort and materials used.
Note: All DNA sequence information should only be submitted as a Word file or Plain Text. Genbank or other published references are not acceptable.
Delivery Materials
5 µg of final construct will be sent
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