The gel extraction system is designed for fast cleanup of DNA fragments from enzymatic PCR reactions and agarose gels. The Retrogen extraction columns contain a silica-gel membrane for binding of up to 10 µg DNA in high-salt buffer and elution in low-salt buffer. Impurities are washed away, and pure DNA is eluted in a small volume of water, ready for use in any subsequent application. The purification procedure removes nucleotides, enzymes, mineral oil, salts, agarose, polyacrylamide, ethidium bromide, dyes, detergents and other impurities from DNA samples. Retrogen silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Binding buffers promote selective adsorption of DNA molecules within the size range of 70 bp to 10 kb.
Key Benefits
Efficient removal of contaminants
Up to 95% recovery of ready-to-use DNA
DNA cleanup in 3 simple steps: Bind, Wash, and Elute
No resins, no phenol, no alcohol precipitation
DNA Recovery from RetroPrep Gel Extraction Kit
The purity and yield of DNA following cleanup is illustrated in Figure 1 below. DNA recovery from agarose gel is typically 60-90%. Up to 10µg DNA in a maximum of 400mg agarose can be processed per gel extraction column.
1-800-RETROGEN
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