Retrogen retroviral vectors are Moloney Murine Leukemia Virus (MoMuLV)-based vectors. We have constructed a foundation vector, pRT-X, which has been trimmed to contain the minimal amount of retroviral sequence required to produce an infectious, replication deficient, retrovirus. A minimized retroviral vector allows the insertion of larger cDNAs of interest. All vectors contain the CMV promoter/enhancer fused to a minimal fragment of the 5'LTR for high level transcription initiation and retroviral genome synthesis. A modified packaging region was constructed to add the wild-type MoMuLV splice acceptor sequence. The addition of the splice acceptor increases mRNA stability and the expression of the inserted gene. Using the 293 gag-pol cell line retroviral packaging system, high titers of viral particles can be routinely obtained with all pRT-X-based vectors. We provide the MoMuLV envelope gene expressed from the CMV promoter/enhancer for high level envelope expression and use in ecotropic infections. Retroviral particles containing an ecotropic envelope are able to infect mouse and rat, but not human cells. For amphotropic envelope expression, we provide the 4070A MuLV envelope gene expressed from the CMV promoter/enhancer vector. Amphotropic envelope containing particles can infect cells of most animal species except hamsters.
Key Benefits
pRT-X is the minimal retroviral vector devoid of any internal gene sequences. This vector may be used as a retroviral expression vehicle where drug selection to produce stable cell lines is not required. For the pRT-X vector, we have inserted the b-galactosidase or luciferase genes for use as positive controls.
1-800-RETROGEN
WELCOME, GUEST
