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Oligonucleotides
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Oligo Synthesis
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Ordering
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Quality Assurance
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Modifications
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Purifications
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Scale and Purity
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DNA Sequencing
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Custom Gene Synth
Oligos - Scale & Purity
Recommended Scale of Synthesis and Purification Levels
Application
Scale of Synthesis
Purification
Antisense studies
1 µM
RP1
End labelling
.2 µM
HPLC, PAGE
Gel shift assay
.2 µM
HPLC, PAGE
Gene synthesis
.2 µM
PAGE
Hybridization
.2 µM
Desalt, RP1
Kinasing
.2 µM
HPLC, PAGE
Mutagenesis
.5 µM, .2 µM
HPLC, PAGE
PCR
.5 nM, .2 µM
Desalted
Qualitative PCR
.5 µM, .2 µM
HPLC, PAGE
RT-PCR
.5 µM, .2 µM
Desalt
Sequencing
.5 µM, .2 µM
Desalt
Modified bases and chemical linkers
.5 µM, .2 µM
RP1
Reporter groups (biotin, DIG or fluorescent dyes)
.5 µM, .2 µM
RP1, HPLC